Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum
نویسندگان
چکیده
منابع مشابه
Morphological and Molecular Differentiation of the Pectinase Producing Fungi Penicillium Expansum and Penicillium Griseoroseum
Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers ...
متن کاملProduction of patulin by Penicillium expansum.
Twenty-seven isolates of Penicillium expansum Lk. ex Thom obtained from Europe, Australia, and North America from seven different fruit hosts all produced patulin in culture. Six isolates were essentially nonpathogenic in apple fruits. In culture, patulin generally accumulated to much higher levels than in apple fruits. At all temperatures permitting fungus growth, patulin was produced. However...
متن کاملPatulin Accumulation In Apples During Storage by Penicillium Expansum and Penicillium Griseofulvum Strains
A part of apples destined to juice production is generally of poor quality. Apples from cold storage or recently harvest (ground harvested or low quality apples) are stored under ambient conditions until they are processed. Since Penicillium expansum and P. griseofulvum are the principal fungal species isolated from stored apples in Brazil, the objective of this study was to investigate the abi...
متن کاملPatulin production in apples decayed by Penicillium expansum.
Sixty isolates of Penicillium expansum were tested for patulin production in decaying apples. All the isolates were found to produce the mycotoxin patulin as determined by thin-layer chromatography. Since patulin is known to be stable in many apple products, the results indicate that apple products made partially from apples decayed by P. expansum will contain patulin which may present a health...
متن کاملThe effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link.
AIMS To study the influence of culture preservation methods and culture conditions on the production of the mycotoxins patulin and citrinin by Penicillium expansum. METHODS AND RESULTS Ten strains of Penicillium expansum were preserved using subculture and maintenance at 4 degrees C, mineral oil, drying on silica gel and freeze-drying. Patulin and citrinin production was assessed on yeast ext...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2007
ISSN: 1517-8382
DOI: 10.1590/s1517-83822007000100011